Bielefeld University Department of Chemistry Biophysical Chemistry and Photochemistry deutsch


Bioanalysis and Protein Biochemistry

Bioanalysis

- analytical gel filtration (also under illumination)

- cofactor identification using UV/Vis and fluorescence spectroscopy

- polysaccharide analysis via IR spectroscopy and electrophoresis (FACE)

- quantification of cellular components with IR spectroscopy

Protein Biochemistry

For biophysical investigations, we overexpress enzymes and photoreceptors or segments thereof in larger amounts in E. coli
(such as the cryptochrome, see J. Biol. Chem. 2007)
.

- cloning, transformation

- overexpression, cell lysis by French press

- purification via affinity chromatography and gel filtration

- quality control by SDS-PAGE and Western Blot

- phosphorylation assays




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