We developped an advanced RP production and secretion system for the green unicellular model alga Chlamydomonas reinhardtii. The versatile expression vector employs the secretion signal of the C. reinhardtii carbonic anhydrase for efficient RP secretion into the culture medium. Unique restriction sites allow fast and easy sub-cloning of sequences of interest. Positive transformants can rapidly be identified by high-throughput plate-level screens via a coupled Gaussia luciferase marker. Compared to the native secretion signal of the Gaussia luciferase, up to 84% higher RP production could be achieved. The target RP is found exclusively in culture medium and can therefore easily be isolated and purified.