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Lehrstuhl für Biochemie und Physiologie der Pflanzen
Fakultät für Biologie W5-117
Universität Bielefeld
Universitätsstraße 25
33615 Bielefeld
Tel: ++49-(0)521-106-5594
Fax: ++49-(0)521-106-6039
E-mail: dortje.golldack@uni-bielefeld.de
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Education and research experience:
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This section describes projects I have been working on. I am particularly interested in molecular biology, biochemistry,
and physiology of abiotic stress in plants. I am working with plant species as Mesembryanthemum crystallinum, Arabidopsis
thaliana, and rice.
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Comparative analysis of gene expression in Arabidopsis thaliana and related halotolerant species.
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Salt stress is a major environmental factor that severely impairs growth and productivity of plants.
Salinity-induced transcript expression is monitored in the salt-sensitive Arabidopsis thaliana and in related
salt-tolerant species by hybridization of cDNA-arrays.
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Expression of a cDNA homologous to AKT1-type potassium channels in rice varieties.
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control
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stress
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The salt-sensitive rice line IR29 and the salt-tolerant rice line Pokkali
accumulate sodium and potassium differently during a short-term stress
of sodium chloride. Pokkali uses ion exclusion while IR29 accumulates
significantly more sodium. Based on this physiological analysis, the
expression of potassium uptake systems was investigated. A rice-cDNA
clone was obtained that shows homology to AKT-type potassium channels
from Arabidopsis. The expression of the transcript was studied
in the salt-tolerant and in the salt-sensitive line.
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Characterization of a high-affinity potassium transporter homologue in rice.
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control
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stress
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In the abscence of potassium in the nutrition medium, Pokkali increasingly
accumulates sodium similar to IR29. A rice cDNA clone homologous to the
high affinity potassium uptake transporter HKT1 from wheat was
obtained. Expression analyses showed a strong decrease of the
transcript in the root differentiation zone of rice plants exposed to
salt stress. The function of the transcript was studied by expression
in yeast and in Xenopus oocytes.
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Salt-inducible signal transduction elements in rice.
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IR29,
stress
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Pokkali,
stress
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Differences in perception and signal transduction might play an important role in the different ion uptake
behaviour of the rice lines IR29 and Pokkali. By cDNA-array hybridizations, we identified salt-inducible signal
transduction elements. We generate transgenic rice plants over-expressing these signalling elements.
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Analysis of AKT-type potassium channels in Mesembryanthemum crystallinum.
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stress
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stress
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Cell and tissue specificity of a putative potassium channel was studied
in M. crystallinum.
The accumulation of the transcript was
analyzed in plants exposed to control conditions and to salt stress.
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Stress-dependent expression of water channel proteins (MIP) in Mesembryanthemum crystallinum.
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stress
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stress
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Major intrinsic proteins (MIP), also termed aquaporins, are supposed to
function as water channels. Regulation of these proteins might be
involved in salt stress resistance of the facultative halophyte M.
crystallinum. Cell specificity and salt stress-dependence of the
expression of MIP in the common ice plant were investigated.
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Effects of salt stress on the expression of different subunits of the vacuolar H+-ATPase.
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stress
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stress
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The vacuolar type H+-ATPase generates proton-motive force for many
secondary active transport processes at the tonoplast. In M.
crystallinum, the V-ATPase is supposed to be the primary proton
pump energizing sodium sequestration into the vacuole. In this project,
the molecular organization and tissue specificity of V-ATPase subunit
expression is studied in M. crystallinum and A. thaliana.
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- Diédhiou, C.J., Popova, O.V., Dietz, K.-J., Golldack, D. (2008).
The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive
gene expression in rice. BMC Plant Biology 8, 49.
- Seidel, T., Schnitzer, D., Golldack, D., Sauer, M., Dietz, K.-J. (2008).
Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis.
BMC Cell Biology 9, 28.
- Diédhiou, C.J., Popova, O.V., Dietz, K.-J., Golldack, D. (2008).
The SUI-homologous translation initiation factor eIF-1 is involved in the regulation of ion
homeostasis in rice. Plant Biology 10, 298-309.
- Wei, W., Alexandersson, E., Golldack, D., Miller, A.J., Kjellbom, P.O., Fricke, W. (2007).
HvPIP1;6, a barley (Hordeum vulgare L.) plasma membrane water channel particularly expressed in
growing compared with non-growing leaf tissues. Plant & Cell Physiology 48, 1132-1147.
- Kader, A.M., Lindberg, S. Seidel, T., Golldack, D., Yemelyanov, V. (2007).
Sodium sensing induces different changes in free cytosolic calcium concentration and pH
in salt-tolerant and -sensitive rice (Oryza sativa) cultivars. Physiologia Plantarum 130, 99-111.
- Hanitzsch, M., Schnitzer, D., Seidel, T. Golldack, D., Dietz, K.-J. (2007).
Transcript level regulation of the vacuolar H+-ATPase subunit isoforms
VHA-a, VHA-E and VHA-G in Arabidopsis thaliana. Molecular Membrane Biology 5-6, 507-518.
- Popova, O.V., Golldack, D. (2007). In the halotolerant Lobularia
maritima (Brassicaceae) salt adaptation correlates with activation of the
vacuolar H+-ATPase and the vacuolar Na+/H+ antiporter. Journal of Plant
Physiology 164, 1278-1288.
- Baier, M., Kandlbinder, A., Dietz, K.-J., Golldack, D. (2007).
Subcellular sites of environmental sensing. Progress in Botany 69, 201-230.
- Kader, A.M., Seidel, T., Golldack, D., Lindberg, S. (2006). Expressions
of OsHKT1, OsHKT2 and OsVHA are differentially regulated under NaCl stress
in salt-sensitive and salt-tolerant rice (Oryza sativa L.) cultivars.
Journal of Experimental Botany 57, 4257-68.
- Diédhiou, C.J., Golldack, D. (2006).Salt-dependent regulation of
chloride channel transcripts in rice. Plant Science 170, 793-800.
- Baier, M., Kandlbinder, A., Dietz, K.-J., Golldack, D. (2006).
New insights into abiotic stress signalling in plants. Progress in
Botany 67, 248-274.
- Seidel, T., Golldack, D., Dietz, K.-J. (2005). Mapping of C-termini of
V-ATPase subunits by in vivo-FRET measurements. FEBS Letters 579,
4374-4382.
- Baier, M., Kandlbinder, A., Golldack, D., Dietz, K.-J. (2005).
Oxidative stress and ozone: mechanisms and responses. Plant, Cell
and Environment 28, 1012-1020.
- Seidel, T., Kluge, C., Hanitzsch, M., Roß, J., Sauer, M.,
Dietz, K.-J., Golldack, D. (2004). Colocalization and
FRET-analysis of subunits c and a of the vacuolar H+-ATPase in
living plant cells. Journal of Biotechnology 112, 165-75.
- Kluge, C., Seidel, T., Bolte, S., Satiat-Jeunemaitre, B.,
Hanitzsch, M., Roß, J., Sauer, M., Golldack, D., Dietz, K.-J.
(2004). Subcellular distribution of the V-ATPase complex in plant
cells, and in vivo localisation of the 100 kDa subunit VHA-a
within the complex. BMC Cell Biology 5, 29, 1-18.
- Golldack, D. (2004). Molecular responses of halophytes to
high salinity. Progress in Botany 65, 219- 234.
- Kluge, C., Lahr, J., Hanitzsch, M., Bolte, S., Golldack, D.,
Dietz K.-J. (2003). New insight into the structure and regulation
of the plant vacuolar H+-ATPase. Journal of Bioenergetics and
Biomembranes 35, 377-388.
- Popova, O.V., Dietz, K.J., Golldack, D. (2003). Salt-dependent
expression of a nitrate transporter and two amino acid transporter
genes in Mesembryanthemum crystallinum. Plant Molecular
Biology 52, 569-578.
- Su, H., Balderas, E., Vera-Estrella, R., Golldack, D., R.,
Quigley, F., Zhao, C., Pantoja, O., Bohnert, H.J. (2003).
Expression of the cation transporter McHKT1 in a halophyte.
Plant Molecular Biology 52, 967-980.
- Golldack, D., Quigley, F., Michalowski, C.B., Kamasani, U.R.,
Bohnert, H.J. (2003). Salinity stress-tolerant and -sensitive rice
(Oryza sativa L.) regulate AKT1-type potassium channel transcripts
differently. Plant Molecular Biology 51, 71-81.
- Kluge, C., Lamkemeyer, P., Tavakoli, N., Golldack, D., Kandlbinder,
A., Dietz, K.-J. (2003). cDNA cloning of 12 subunits of the V-type
ATPase from Mesembryanthemum crystallinum and their expression
under stress. Molecular Membrane Biology 20, 171-183.
- Golldack, D., Vera, P., Dietz, K.-J. (2003). Expression of
subtilisin-like serine proteases in Arabidopsis thaliana is
cell-specific and responds to jasmonic acid and heavy metals with
developmental differences. Physiologia Plantarum 118, 64-73.
- Golldack, D., Su, H., Quigley, F., Kamasani, U.R., Muñoz-Garay,
C., Balderas, E., Popova, O.V., Bennett, J., Bohnert, H.J.,
Pantoja, O. (2002). Characterization of a HKT-type transporter
in rice as a general alkali cation transporter.
The Plant Journal 31, 529-542.
- Su, H., Golldack, D., Zhao, C., Bohnert, H.J. (2002). The
expression of HAK-type K+ transporters is regulated in response
to salinity stress in common ice plant. Plant Physiology 129,
1482-1493.
- Popova, O.V., Ismailov, S.F., Popova, T.N., Dietz, K.-J.,
Golldack, D. (2002). Salt-induced expression of NADP-dependent
isocitrate dehydrogenase and ferredoxin-dependent glutamate
synthase in Mesembryanthemum crystallinum. Planta 215, 906-913.
- Golldack, D., Popova, O.V., Dietz, K.-J. (2002). Mutation
of the matrix metalloproteinase At2-MMP inhibits growth and
causes late flowering and early senescence in Arabidopsis. The
Journal of Biological Chemistry 277, 5541-5547.
- Dietz, K.-J., Golldack, D. (2002). Crassulacean acid
metabolism, a special case of pH regulation and H+-fluxes.
In: Handbook of plant growth: pH as master variable in plant
growth. Z. Rengel, ed., Marcel Dekker, New York.
- Tavakoli, N., Kluge, C., Golldack., D., Mimura, T.,
Dietz, K.-J. (2001). Reversible redox control of plant vacuolar
H+-ATPase activity is related to disulfide bridge formation in
subunit E as well as subunit A. The Plant Journal 28, 51-59.
- Golldack, D., Dietz, K.-J. (2001). Salt-induced expression
of the vacuolar H+-ATPase in the common ice plant is developmentally
controlled and tissue specific. Plant Physiology 125, 1643-1654.
- Su, H., Golldack, D., Katsuhara, M., Zhao, C., Bohnert, H.J. (2001).
Expression and stress-dependent induction of potassium channel
transcripts in the common ice plant. Plant Physiology 125, 604-614.
- Dietz, K.-J., Tavakoli, N., Kluge, C., Mimura, T., Sharma, S.,
Harris, G.C., Chardonnens, A., Golldack, D. (2001). Significance
of the V-type ATPase for the adaptation to stressful growth conditions
and its regulation on the molecular and biochemical level. Journal
of Experimental Botany 52, 1969-1980.
- Kirch, H.H., Vera-Estrella, R., Golldack, D., Quigley, F., Michalowski,
C.B., Barkla, B.J., Bohnert, H.J. (2000). Expression of water
channel proteins in Mesembryanthemum crystallinum. Plant
Physiology 123, 111-124.
- Tavakoli, N., Eckerskorn, C., Golldack, D., Dietz, K.-J. (1999).
Subunit C of the vacuolar H+-ATPase of Hordeum vulgare.
FEBS Letters 456, 68-72.
- Kluge, C., Golldack, D., Dietz, K.-J. (1999). Subunit D of
the vacuolar H+-ATPase of Arabidopsis thaliana. Biochimica et
Biophysica Acta 1419, 105-110.
- Bohnert, H.J., Golldack, D., Ishitani, M., Kamasani, U.R.,
Rammesmayer, G., Shen, B., Sheveleva, E., Jensen, R.G. (1999).
Salt tolerance engineering - which are the essential mechanisms?
In: G.S. Singhal, G. Renger, S.K. Sopory, K.-D. Irrgang, and
Govindjee (Ed.) Concepts in photobiology: photosynthesis and
photomorphogenesis. Narosa Publishing House, New Delhi.
- Golldack, D., Kamasani, U.R., Quigley, F., Bennett, J.,
Bohnert, H.J. (1997). Salt-stress dependent expression of a
HKT1-type high affinity potassium transporter in rice. Plant
Physiology S. 114, 118.
- Su, H., Golldack, D., Bohnert, H.J. (1997). Characterization
of a putative potassium transporter in Mesembryanthemum crystallinum.
Plant Physiology S. 114, 116.
- Bohnert, H.J., Golldack, D., Ishitani, M., Kamasani, U.R.,
Rammesmayer, G., Shen, B., Sheveleva, E., Jensen, R.G. (1996).
Salt tolerance engineering requires multiple gene transfers.
Annals of the New York Academy of Sciences 115-125.
- Geib, K., Golldack, D., Gimmler, H. (1996). Is there a
requirement for an external carbonic anhydrase in the extremely
acid resistant green alga Dunaliella acidophila? European Journal
of Phycology 31, 273-284.
- Golldack, D., Dietz, K.-J., Gimmler, H., (1995). The effects
of sudden salt stress on protein synthesis in the green alga
Dunaliella parva. Journal of Plant Physiology 146, 508-514.
- Golldack, D., Dietz, K.-J., Gimmler, H. (1995). Salt-dependent
protein composition in the halotolerant green alga Dunaliella parva.
Botanica Acta 108, 227-232.
- Hagemann, M., Golldack, D., Biggins, J., Erdmann, N. (1993).
Salt-dependent protein phosphorylation in the cyanobacterium
Synechocystis PCC 6803. FEMS Microbiology Letters 113, 205-210.
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Diplomarbeiten und Laborübungen
(für Studenten der Universität Bielefeld):
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Wir interessieren uns für die molekulare Charakterisierung der Anpassung von Pflanzen an den Umweltfaktor Salz und
für die pflanzliche Entwicklung unter Salzstressbedingungen. Im Rahmen unserer laufenden Projekte können wir
jederzeit interessante Themen für Diplomarbeiten und Laborübungen anbieten.
Bei Interesse an einer Diplomarbeit, einer Abschlussarbeit oder Laborübungen wenden Sie sich bitte an mich:
E-mail: dortje.golldack@uni-bielefeld.de,
Telefon: 106 5594, oder kommen Sie in mein Büro in W5-117.
In unseren Arbeiten setzen wir molekularbiologische, physiologische und biochemische Methoden ein.
Hierzu gehören z.B. die folgenden Methoden:
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cDNA-Array-Hybridisierungen |
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Herstellung und Charakterisierung von transgenen Pflanzen (Arabidopsis, Reis) |
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rekombinante DNA-Techniken, Northern-, Western-, Southern-Blotting |
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In situ-Hybridisierung und In situ-PCR |
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Fluoreszenzmikroskopie und konfokale Laserscanning-Mikroskopie von Reportergenen
in lebenden Pflanzenzellen (GFP, YFP, CFP) |
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heterologe Expression von pflanzlichen Proteinen (z.B. in Hefe) |
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Projects