Department of Chemistry
 
 
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Universität Bielefeld > Department of Chemistry
  

Auszeichnungen / 
Auszeichnungen

Auszeichnungen für hervorragende Masterarbeiten in Biochemie und Chemie

Die beiden Masterabsolventen Christian Bartz (Biochemie) und Florian Venghaus (Chemie) der Universität Bielefeld wurden jetzt für ihre hervorragenden Masterabschlüsse im Jahr 2015 mit einem Förderpreis in Höhe von jeweils 2.000 Euro ausgezeichnet. [mehr...]


Current work from our research groups 


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The two-component regulatory system PhoR/PhoB induces the expression of several genes in response to phosphate starvation in Escherichia coli. In order to quantify these protein-DNA interactions and to study the time-resolved dynamics of the binding mechanism, the specific recognition of different oligonucleotide duplexes by the DNA-binding domain of PhoB (PhoBDBD) was analyzed using surface plasmon resonance. In addition the two point mutants PhoBDBDD196A and PhoBDBDR219A were obtained and the DNA recognition in comparison to the wildtype PhoBDBD was investigated. Aspartic acid 196 and arginine 219 mediate specific minor groove interactions. All results reveal that at high PhoBDBD-concentrations all recognition sequences of the pho box are occupied. Decreasing the protein amount results in a mixture of free oligonucleotides and DNA molecules occupied by two WT-PhoBDBD. Moreover, the SPR results indicate that both binding site segments, the TGTCA-motif and the A/T-rich minor groove, are essential for the binding process. A comparison of different regulons additionally proved the dependency of the recognition process on the base composition of the minor groove.
M. Ritzefeld, K. Wollschläger , G. Niemann , D. Anselmetti and N. Sewald, Mol. BioSyst., 2011, 7, 3132–3142.


Alle GDCh-Vorträge

International Bunsen
Discussion Meetings:
CdCC 2017 Neutrons in Chemistry
25.07.2016

CdCC 2017 Chemistry and Diagnostics for Clean Combustion
21.06.2017

CM2


Magicbullet European Training Network MAGICBULLET


Core Facility



FOR 945

Nanomagnete: von der Synthese über die Wechselwirkung mit Oberflächen zur Funktion