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Universität Bielefeld > Department of Chemistry
  

CHE-Ranking /  Auszeichnungen

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Ein Chemiestudium an der Universität Bielefeld zeichnet sich durch hohe Qualität in Lehre, Betreuung und Ausstattung aus. Wir sind stolz auf das Urteil unserer Studierenden im aktuellen CHE-Ranking: Die Bielefelder Fakultät für Chemie befindet sich hier unter den 5 besten Universitäten Deutschlands!
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Auszeichnungen

Auszeichnungen für hervorragende Masterarbeiten in Biochemie und Chemie

Die beiden Masterabsolventen Christian Bartz (Biochemie) und Florian Venghaus (Chemie) der Universität Bielefeld wurden jetzt für ihre hervorragenden Masterabschlüsse im Jahr 2015 mit einem Förderpreis in Höhe von jeweils 2.000 Euro ausgezeichnet. [mehr...]


Current work from our research groups 


Biochemistry

Cooperative Binding of PhoBDBD to Its Cognate DNA Sequence—A Combined Application of Single–Molecule and Ensemble Methods

A combined approach based on isothermal titration calorimetry (ITC), fluorescence resonance energy transfer (FRET) experiments, circular dichroism spectroscopy (CD), atomic force microscopy (AFM) dynamic force spectroscopy (DFS), and surface plasmon resonance (SPR) was applied to elucidate the mechanism of protein—DNA complex formation and the impact of protein dimerization of the DNA–binding domain of PhoB (PhoBDBD). These insights can be translated to related members of the family of winged helix–turn– helix proteins. One central question was the assembly of the trimeric complex formed by two molecules of PhoBDBD and two cognate binding sites of a single oligonucleotide. In addition to the native protein WT–PhoBDBD, semisynthetic covalently linked dimers with different linker lengths were studied. The ITC, SPR, FRET, and CD results indicate a positive cooperative binding mechanism and a decisive contribution of dimerization on the complex stability. Furthermore, an alanine scan was performed and binding of the corresponding point mutants was analyzed by both techniques to discriminate between different binding types involved in the protein—DNA interaction and to compare the information content of the two methods DFS and SPR. In light of the published crystal structure, four types of contribution to the recognition process of the pho box by the protein PhoBDBD could be differentiated and quantified. Consequently, it could be shown that investigating the interactions between DNA and proteins with complementary techniques is necessary to fully understand the corresponding recognition process.

Markus Ritzefeld, Volker Walhorn, Christin Kleineberg, Adeline Bieker, Klaus Kock, Christian Herrmann, Dario Anselmetti, Norbert Sewald, Biochemistry, 2013, 52, 8177–8186 PUB


Alle GDCh-Vorträge

International Bunsen
Discussion Meetings:
CdCC 2017 Neutrons in Chemistry
25.07.2016

CdCC 2017 Chemistry and Diagnostics for Clean Combustion
21.06.2017

CM2


Magicbullet European Training Network MAGICBULLET


Core Facility



FOR 945

Nanomagnete: von der Synthese über die Wechselwirkung mit Oberflächen zur Funktion