Bielefeld University Department of Chemistry Biophysical Chemistry and Photochemistry deutsch


Biocatalysis and Bioanalysis

Biocatalysis

Regioselective halogenation of indoles can be performed under mild conditions using biocatalysis. We have established a simple procedure for light-driven biocatalysis, which makes several auxiliary enzymes redundant (see ChemCatChem 2018).

- green chemistry: requires light, air and NaCl

- less formation of H2O2 by uncoupling reaction

- single-enzyme reaction facilitates engineering



Bioanalysis and Protein Biochemistry

- analytical gel filtration (also under illumination)

- cofactor identification using UV/Vis and fluorescence spectroscopy

- polysaccharide analysis via IR spectroscopy and electrophoresis (FACE)

- quantification of cellular components with IR spectroscopy

For biophysical investigations, we overexpress enzymes and photoreceptors or segments thereof in larger amounts in E. coli
(such as the cryptochrome, see J. Biol. Chem. 2007)
.

- mutagenesis, transformation

- overexpression, cell lysis by French press

- global and selective isotope labeling

- purification via affinity chromatography and gel filtration

- quality control by SDS-PAGE and Western Blot

- phosphorylation assays







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