Bielefeld University Department of Chemistry Biophysical Chemistry and Photochemistry deutsch

Refereed Publications


  • Gilzer, D., Hillmann, R., Goett-Zink, L., Klocke, J. L., Viefhues, M., Anselmetti, D., and Kottke, T.*
    "Biphasic Formation of 2D Nanomembranes by Photopolymerization of Diacetylene Lipids as Revealed by Infrared Difference Spectroscopy",
    Langmuir 35, 9343-9351.
  • Oldemeyer, S.,* Mittag, M., and Kottke, T.*
    "Time-resolved Infrared and Visible Spectroscopy on Cryptochrome aCRY: Basis for Red Light Reception",
    Biophys. J. 117, 490-499.
  • Wiehemeier, L., Brändel, T., Hannappel, Y., Kottke, T., and Hellweg, T.*
    "Synthesis of Smart Dual-responsive Microgels: Correlation Between Applied Surfactants and Obtained Particle Morphology",
    Soft Matter 15, 5673-5684.
  • Bannister, S., Böhm, E., Zinn, T., Hellweg, T., and Kottke, T.*
    "Arguments for an Additional Long-lived Intermediate in the Photocycle of the Full-length Aureochrome 1c Receptor:
    a Time-resolved Small-angle X-ray Scattering Study",
    Struct. Dyn. 6, 034701.
  • > PDF <

  • Wiehemeier, L., Cors, M., Wrede, O., Oberdisse, J., Hellweg, T., and Kottke, T.*
    "Swelling Behaviour of Core-Shell Microgels in H2O, Analysed by Temperature-Dependent FTIR Spectroscopy",
    Phys. Chem. Chem. Phys. 21, 572-580.
  • Highlighted as HOT ARTICLE by PCCP

  • Blankenburg, L., Schroeder, L., Habenstein, F., Blasiak, B., Kottke, T., and Bredenbeck, J.*
    "Following Local Light-Induced Structure Changes and Dynamics of the Photoreceptor PYP with the Thiocyanate IR Label",
    Phys. Chem. Chem. Phys. 21, 6622-6634.
  • Niermeier, P., Wickemeyer, L., Neumann, B., Stammler, H.-G., Goett-Zink, L., Kottke, T.,* and Mitzel, N. W.*
    "Aurophilicity in Action: Stepwise Formation of Dinuclear Au(I) Macrocycles with Rigid 1,8-Dialkynylanthracenes",
    Dalton T. 48, 4109-4113.
  • Ismail, M., Schroeder, L., Frese, M., Kottke, T., Hollmann, F., Paul, C. E., and Sewald, N.*
    "Straightforward Regeneration of Reduced Flavin Adenine Dinucleotide Required for Enzymatic Tryptophan Halogenation",
    ACS Catal. 9, 1389-1395.
  • > PDF <


  • Ihalainen, J.*, Gustavsson, E., Schroeder, L., Donnini, S., Lehtivuori, H., Isaksson, L., Thöing, C., Modi, V., Berntsson, O., Stucki-Buchli, B., Liukkonen, A., Häkkänen, H., Kalenius, E., Westenhoff, S.*, and Kottke, T.*
    "Chromophore-Protein Interplay During the Phytochrome Photocycle Revealed by Step-Scan FTIR Spectroscopy",
    J. Am. Chem. Soc. 140, 12396-12404.
  • Klocke, J. L., Mangold, M.*, Allmendinger, P., Hugi, A., Geiser, M., Jouy, P., Faist, J., and Kottke, T.
    "Single-Shot Sub-microsecond Mid-infrared Spectroscopy on Protein Reactions with Quantum Cascade Laser Frequency Combs",
    Anal. Chem. 90, 10494-10500.
  • > PDF <

  • Schroeder, L., Frese, M., Müller, C., Sewald, N., and Kottke, T.*
    "Photochemically Driven Biocatalysis of Halogenases for the Green Production of Chlorinated Compounds",
    ChemCatChem 10, 3336-3341.
  • Neubauer, P. R., Widmann, C., Wibberg, D., Schroeder, L., Frese, M., Kottke, T., Kalinowski, J., Niemann, H. H., and Sewald, N.*
    "A Flavin-Dependent Halogenase from Metagenomic Analysis Prefers Bromination over Chlorination",
    PLoS ONE 13, e0196797.
  • Hillmann, R., Viefhues, M., Goett-Zink, L., Gilzer, D., Hellweg, T., Gölzhauser, A., Kottke, T., and Anselmetti, D.*
    "Characterization of Robust and Free-Standing 2D-Nanomembranes of UV-Polymerized Diacetylene Lipids",
    Langmuir 34, 3256-3263.
  • Schroeder, L., Oldemeyer, S., and Kottke, T.*
    "Time-Resolved Infrared Spectroscopy on Plant Cryptochrome - Relevance of Proton Transfer and ATP Binding for Signaling",
    J. Phys. Chem. A 122, 140-147.
  • Kottke, T., Xie, A., Larsen, D. S., and Hoff, W. D.
    "Photoreceptors Take Charge: Emerging Principles for Light Sensing",
    Annu. Rev. Biophys. 47, 291-313.
  • > PDF <

  • Wördenweber, R.,Rokitta, S. D., Heidenreich, E., Corona, K., Kirschhöfer, F., Fahl, K., Klocke, J. L., Kottke, T., Brenner-Weiß, G., Rost, B., Mussgnug, J. H., and Kruse, O.*
    "Phosphorus and Nitrogen Starvation Reveal Life-Cycle Specific Responses in the Metabolome of Emiliania huxleyi (Haptophyta)",
    Limnol. Oceanogr. 63, 203-226.
  • 2017

  • Brändel, T., Wiehemeier, L., Kottke, T., and Hellweg, T.*
    "Microphase Separation of Smart Double-Responsive Copolymer Microgels Studied by Local Fluorescence Probes",
    Polymer 125, 110-116.
  • Kottke, T.*, Oldemeyer, S., Wenzel, S., Zou, Y., and Mittag, M.*
    "Cryptochrome Photoreceptors in Green Algae: Unexpected Versatility of Mechanisms and Functions",
    J. Plant Physiol. 217, 4-14.
  • Sommer, C., Dietz, M. S., Patschkowski, T., Mathes, T., and Kottke, T.*
    "Light-Induced Conformational Changes in the Plant Cryptochrome Photolyase Homology Region Resolved by Selective Isotope Labeling and Infrared Spectroscopy",
    Photochem. Photobiol. 93, 881-887.
  • Zou, Y., Wenzel, S., Müller, N., Prager, K., Jung, E.-M., Kothe, E., Kottke, T., and Mittag, M.*
    "An Animal-Like Cryptochrome Controls the Chlamydomonas Sexual Cycle",
    Plant Physiol. 174, 1334-1347.
  • Müller, N., Zou, Y., Wenzel, S., Künzel, S., Sasso, S., Weiß, D., Prager, K., Grossman, A., Kottke, T., and Mittag, M.*
    "A Plant Cryptochrome Controls Key Features of the Chlamydomonas Circadian Clock and its Life Cycle",
    Plant Physiol. 174, 185-201.
  • Recommended by the Faculty of 1000 (Webb A: 10.3410/f.727464471.793530380)

  • Hahn, K., Hertle, Y., Bloess, S., Kottke, T., Hellweg, T., and Fischer von Mollard, G.*
    "Activation of Recombinantly Expressed L-Amino Acid Oxidase from Rhizoctonia solani by Sodium Dodecyl Sulfate",
    Molecules 22, 2272.
  • > PDF <

  • Dreischmeier, K., Budke, C., Wiehemeier, L., Kottke, T., and Koop, T.*
    "Boreal Pollen Contain Ice-Nucleating as Well as Ice-Binding Antifreeze Polysaccharides",
    Sci. Rep. 7, 41890.
  • > PDF <

  • Kottke, T., Lorenz-Fonfría, V., and Heberle, J.*
    "The Grateful Infrared - Sequential Protein Structural Changes Resolved by Infrared Difference Spectroscopy",
    J. Phys. Chem. B 121, 335-350.
  • Hahn, K., Neumeister, K., Mix, A., Kottke, T., Gröger, H., and Fischer von Mollard, G.*
    "Recombinant Expression and Characterization of a L-Amino Acid Oxidase From the Fungus Rhizoctonia solani",
    Appl. Microbiol. Biotechnol. 101, 2853-2864.
  • König, S., Juhas, M., Jäger, S., Kottke, T., and Büchel, C.*
    "The Cryptochrome - Photolyase Protein Family in Diatoms",
    J. Plant Physiol. 217, 15-19.
  • Kroth, P. G.*, Wilhelm, C., and Kottke, T.
    "An Update on Aureochromes: Phylogeny - Mechanism - Function",
    J. Plant Physiol. 217, 20-26.
  • 2016

  • Petroutsos, D., Tokutsu, R., Maruyama, S., Flori, S., Greiner, A., Magneschi, L., Cusant, L., Kottke, T., Mittag, M., Hegemann, P., Finazzi, G., and Minagawa, J.*
    "A Blue-Light Photoreceptor Mediates the Feedback Regulation of Photosynthesis",
    Nature 537, 563-566.
  • Oldemeyer, S., Franz, S., Wenzel, S., Essen, L.-O., Mittag, M., and Kottke, T.*
    "Essential Role of an Unusually Long-Lived Tyrosyl Radical in the Response to Red Light of the Animal-Like Cryptochrome aCRY",
    J. Biol. Chem. 291, 14062-14071.
  • Banerjee, A. §, Herman, E. §, Serif, M., Reyna, M. M., Hepp, S., Pokorny, R., Kroth, P. G., Essen, L.-O.*, and Kottke, T.
    "Allosteric Communication Between DNA-Binding and Light-Responsive Domains of Diatom Class I Aureochromes",
    Nucleic Acids Res. 44, 5957-5970.
  • § My group contributed as first author

  • Domratcheva, T.*, Hartmann, E., Schlichting, I., and Kottke, T.*
    "Evidence for Tautomerisation of Glutamine in BLUF Blue Light Receptors by Vibrational Spectroscopy and Computational Chemistry",
    Sci. Rep. 6, 22669.
  • > PDF <

  • Banerjee, A., Herman, E., Kottke, T., and Essen, L.-O.*
    "Structure of a Native-like Aureochrome 1a LOV Domain Dimer from Phaeodactylum tricornutum",
    Structure 24, 171-178.
  • 2015

  • Thöing, C., Oldemeyer, S., and Kottke, T.*
    "Microsecond Deprotonation of Aspartic Acid and Response of the α/β Subdomain Precede C-terminal Signaling in the Blue Light Sensor Plant Cryptochrome",
    J. Am. Chem. Soc. 137, 5990-5999.
  • > PDF <

  • Herman, E., and Kottke, T.*
    "Allosterically Regulated Unfolding of the A´α Helix Exposes the Dimerization Site of the Blue-Light-Sensing Aureochrome-LOV Domain",
    Biochemistry 54, 1484-1492.
  • Hense, A., Herman, E., Oldemeyer, S., and Kottke, T.*
    "Proton Transfer to Flavin Stabilizes the Signaling State of the Blue Light Receptor Plant Cryptochrome",
    J. Biol. Chem. 290, 1743-1751.
  • 2014

  • Spexard, M., Thöing, C., Beel, B., Mittag, M., and Kottke, T.*
    "Response of the Sensory Animal-Like Cryptochrome aCRY to Blue and Red Light as Revealed by Infrared Difference Spectroscopy",
    Biochemistry 53, 1041-1050.
  • Juhas, M., von Zadow, A., Spexard, M., Schmidt, M., Kottke, T., and Büchel, C.*
    "A Novel Cryptochrome in the Diatom Phaeodactylum tricornutum Influences the Regulation of Light Harvesting Protein Levels",
    FEBS J. 281, 2299-2311.
  • 2013

  • Thöing, C., Pfeifer, A., Kakorin, S., and Kottke, T.*
    "Protonated triplet-excited flavin resolved by step-scan FTIR spectroscopy: Implications for photosensory LOV domains",
    Phys. Chem. Chem. Phys. 15, 5916-5926.
  • Herman, E., Sachse, M., Kroth, P. G., and Kottke, T.*
    "Blue-Light-Induced Unfolding of the Jα Helix Allows for the Dimerization of Aureochrome-LOV from the Diatom Phaeodactylum tricornutum",
    Biochemistry 52, 3094-3101.
  • 2012

  • Immeln, D., Weigel, A., Kottke, T.*, and Pérez Lustres, J. L.*
    "Primary Events in the Blue Light Sensor Plant Cryptochrome: Intraprotein Electron and Proton Transfer Revealed by Femtosecond Spectroscopy",
    J. Am. Chem. Soc. 134, 12536-12546.
  • Burney, S., Wenzel, R., Kottke, T., Roussel, T., Hoang, N., Bouly, J.-P., Bittl, R., Heberle, J., and Ahmad, M.*
    "Single Amino Acid Substitution Reveals Latent Photolyase Activity in Arabidopsis cry1",
    Angew. Chem. Int. Ed. 51, 9356-9360.
    Angew. Chem. 124, 9490-9494.
  • Beel, B. §, Prager, K. §, Spexard, M. §, Sasso, S., Weiss, D., Müller, N., Heinnickel, M., Dewez, D., Ikoma, D., Grossman, A. R., Kottke, T., and Mittag, M.*
    "A Flavin Binding Cryptochrome Photoreceptor Responds to Both Blue and Red Light in Chlamydomonas reinhardtii",
    Plant Cell 24, 2992-3008.
  • § My group contributed as first author


  • Spexard, M., Immeln, D., Thöing, C., and Kottke, T.*
    "Infrared Spectrum and Absorption Coefficient of the Cofactor Flavin in Water",
    Vib. Spectrosc. 57, 282-287.
  • Bauer, C., Rabl, C.-R., Heberle, J., and Kottke, T.*
    "Indication for a Radical Intermediate Preceding the Signaling State in the LOV Domain Photocycle",
    Photochem. Photobiol. 87, 548-553.
  • 2010

  • Immeln, D., Pokorny, R., Herman, E., Moldt, J., Batschauer, A., and Kottke, T.*
    "Photoreaction of Plant and DASH Cryptochromes Probed by Infrared Spectroscopy: The Neutral Radical State of Flavoproteins",
    J. Phys. Chem. B 114, 17155-17161.
  • Kottke, T.*, van de Linde, S., Sauer, M., Kakorin, S., and Heilemann, M.*
    "Identification of the Product of Photoswitching of an Oxazine Fluorophore Using Fourier Transform Infrared Difference Spectroscopy",
    J. Phys. Chem. Lett. 1, 3156-3159.
  • Thamarath, S. S., Heberle, J., Hore, P. J., Kottke, T.*, and Matysik, J.*
    "Solid-State Photo-CIDNP Effect Observed in Phototropin LOV1-C57S by 13C Magic-Angle Spinning NMR Spectroscopy",
    J. Am. Chem. Soc. 132, 15542-15543.
  • Pfeifer, A., Mathes, T., Lu, Y., Hegemann, P., and Kottke, T.*
    "Blue Light Induces Global and Localized Conformational Changes in the Kinase Domain of Full-Length Phototropin",
    Biochemistry 49, 1024-1032.
  • Selected by the Faculty of 1000 as one of the top 2% of published articles in biology and medicine (Liscum E: 2010.

  • Ataka, K., Kottke, T., and Heberle, J.*
    "Thinner, Smaller, Faster: IR Techniques To Probe the Functionality of Biological and Biomimetic Systems",
    Angew. Chem. Int. Ed. 49, 5416-5424.

    Ataka, K., Kottke, T., and Heberle, J.*
    "Dünner, kleiner, schneller - wie die IR-Spektroskopie zur Aufklärung des Funktionsmechanismus biologischer und biomimetischer Systeme beiträgt",
    Angew. Chem. 122, 5544-5553.
  • 2003-3009

  • Langenbacher, T., Immeln, D., Dick, B., and Kottke, T.* (2009)
    "Microsecond Light-induced Proton Transfer to Flavin in the Blue Light Sensor Plant Cryptochrome",
    J. Am. Chem. Soc. 131, 14274-14280.
  • Maag, D., Kottke, T., Schulte, M., and Godt, A.* (2009)
    "Synthesis of Monodisperse Oligo(1,4-phenyleneethynylene-alt-1,4-triptycyleneethynylene)s",
    J. Org. Chem. 74, 7733-7742.
  • Bahlawane, N.*, Ngamou, P. H. T., Vannier, V., Kottke, T., Heberle, J., and Kohse-Höinghaus, K. (2009)
    "Tailoring the properties and the reactivity of the spinel cobalt oxide",
    Phys. Chem. Chem. Phys. 11, 9224-9232.
  • Pfeifer, A., Majerus, T., Zikihara, K., Matsuoka, D., Tokutomi, S., Heberle, J., and Kottke, T.* (2009)
    "Time-Resolved Fourier Transform Infrared Study on Photoadduct Formation and Secondary Structural Changes within the Phototropin LOV Domain",
    Biophys. J. 96, 1462-1470.
  • Immeln, D., Schlesinger, R., Heberle, J., and Kottke, T.* (2007)
    "Blue Light Induces Radical Formation and Autophosphorylation in the Light-sensitive Domain of Chlamydomonas Cryptochrome",
    J. Biol. Chem. 282, 21720-21728.
  • Majerus, T., Kottke, T., Laan, W., Hellingwerf, K., and Heberle, J.* (2007)
    "Time-resolved FT-IR spectroscopy traces signal relay within the blue-light receptor AppA",
    ChemPhysChem 8, 1787-1789.
  • Berndt, A., Kottke, T., Breitkreuz, H., Dvorsky, R., Hennig, S., Alexander, M., and Wolf, E.* (2007)
    "A Novel Photoreaction Mechanism for the Circadian Blue-Light Photoreceptor Drosophila Cryptochrome",
    J. Biol. Chem. 282, 13011-13021.
  • Kottke, T., Hegemann, P., Dick, B., and Heberle, J.* (2006)
    "The Photochemistry of the Light-, Oxygen-, and Voltage-Sensitive Domains in the Algal Blue Light Receptor Phot",
    Biopolymers 82, 373-378.
  • Kottke, T.*, Batschauer, A., Ahmad, M., and Heberle, J. (2006)
    "Blue-Light-Induced Changes in Arabidopsis Cryptochrome 1 Probed by FTIR Difference Spectroscopy",
    Biochemistry 45, 2472-2479.
  • Guo, H., Kottke, T., Hegemann, P., and Dick, B.* (2005)
    "The Phot LOV2 Domain and its Interaction with LOV1",
    Biophys. J. 89, 402-412.
  • Losi, A.*, Kottke, T., and Hegemann, P. (2004)
    "Recording of blue light-induced energy and volume changes within the wild-type and mutated phot-LOV1 domain from Chlamydomonas reinhardtii",
    Biophys. J. 86, 1051-1060.
  • Kottke, T.*, Dick, B., Fedorov, R., Schlichting, I., Deutzmann, R., and Hegemann, P. (2003)
    "Irreversible Photoreduction of Flavin in a Mutated Phot-LOV1 domain",
    Biochemistry 42, 9854-9862.
  • Kottke, T.*, Heberle, J., Hehn, D., Dick, B., and Hegemann, P. (2003)
    "Phot-LOV1: Photocycle of a Blue-Light Receptor Domain from the Green Alga Chlamydomonas reinhardtii",
    Biophys. J. 84, 1192-1201.

  • *corresponding author

Proceedings and Unrefereed Publications

  • Hillmann, R., Gilzer, D., Knust, S., Biere, N., Viefhues, M., Toensing, K., Kottke, T., and Anselmetti, D. (2017)
    "Preparation of 2D Phospholipid and Copolymer Nanomembranes",
    Mater. Today: Proc. 4, S87-S92.
  • Beel, B., Müller, N., Kottke, T., and Mittag, M. (2013)
    "News about cryptochrome photoreceptors in algae",
    Plant Sig. Behav. 8, 1-4.
  • Kottke, T. (2012)
    "An den Grenzen der Sensitivität der FT-IR-Spektroskopie: Die Dynamik von biologischen Blaulichtsensoren",
    Bunsen-Magazin 14, 38-43.
    > PDF <
  • Kottke, T. (2011)
    "Durch Infrarotspektroskopie von einem statischen zu einem dynamischen Bild der Photochemie von sensorischen Blaulichtrezeptoren",
    Habilitation treatise, Bielefeld University, Germany.
  • Pfeifer, A., and Kottke, T. (2011)
    "The big exception: flavin-blue-light sensors",
    Nachr. Chem. 59, 23-28.
    > PDF <
  • Kottke, T. (2003)
    "Spektroskopische Aufklärung des Photozyklus der LOV1-Domäne eines Phot-Blaulichtrezeptors",
    PhD thesis, University of Regensburg, Germany.

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