Plant Biochemistry and Physiology

Proton-pumping V-ATPase functions as molecular motor and is the primary electrogenic pump at endomembranes including endoplasmatic reticulum, Golgi apparatus, various vesicles and vacuoles. It consists of many different subunits and structurally of the rotor, the central stalk, one or more peripheral stalks and the head. Following detailed in vivo interaction studies using FRET, we now focus on regulation, assembly and interaction with neighboring transporters. Thereby we seek an integrated understanding of V-ATPase activity for endomembrane functionality.