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  • LiMiTec

    Light Microscopy Technology Platform of Bielefeld University

    © Universität Bielefeld

Light Microscopy Technology Platform

Welcome to the LiMiTec the Light Microscopy Technology Platform of Bielefeld University.The LiMiTec started as an intiative of the Faculty of Biology and now represents a state-of-the-art imaging facility bundling all light microscopes of the Faculty of Biology. The group of Thomas Huser from the Faculty of Physics provides a strong expertise in Raman microscopy and super resolution microscopy. For technical support please contact Dr. Thorsten Seidel or use the contact form.

News

Corona virus & Covid-19: Starting at  December 16th 2020, only basal processes are maintained at Bielefeld University again. Please contact Thorsten before starting new experiments and get an introduction to desinfection of microscopes.

LiMiTec is open again. Please desinfect the microscopes before and after our experiments. Due to the limitation to basal processes, the microscopes have been rarely used within the last days. If possible, coordinate your bookings, but keep distance when you handover a system.


Guidelines

Before starting to use any microscope of the LiMiTec, an introduction to the microscope is mandatory and safety instructions according to GenTSV (safety level S1) are required. Keep the labs clean and pay attention to the waste separation.

For booking the confocal microscopes, request a login once you have been introduced to the microscope. The booking platform is available at http://web.biologie.uni-bielefeld.de/limitec-booking/. Booking is possible for up to two weeks in advance. Do not exceed four hours for a single slot and three slots a week Monday to Friday from 8:00 to 18:00, long term experiments should be performed at the weekend or overnight. Avoid gaps between slots. Delete your booking, once it turns out that you are not going to use it. If that happens at short notice, inform other users who booked the microscope that day by email. Keep yourself updated about the users who booked the microscope before and after your slot to ensure proper handover of the system.

Before you start, contact the one who booked it before you to avoid gaps and unnecessary switching off of the HBO/HXP-lamps and argon-ion lasers, if they are still needed. Check the equipment and the logbook for obvious damages and reported problems. Report problems in the logbook and also inform the staff. Before you leave, clean all objective lenses you have used. Keep the lab clean and contact the following user. If you are the last user, ensure that the system is properly shut down. If you are the second last user, check if the last user that day shows up.

Copy your data to the servers of the faculty and check the files. Delete the local files the next time you are using the microscope. There is no backup of local files. Data of people who are no longer affiliated with Bielefeld University will be deleted. The same applies for the image processing PC in the isotope lab.

The following methods are frequently applied:


Localisation of proteins

Tissue specificity

subcellular localisation

Co-localisation

Protein Dynamics

Tracking of proteins

FRAP

FLIP

Protein-Interactions

BiFC

FRET

Measurement of physiological parameters

Redoxpotential: Dichlorofluorescein, HyPer, roGFP

Vacuolar pH: 6-Carboxyfluorescein

Login for the booking process:

http://web.biologie.uni-bielefeld.de/limitec-booking/

Please select an instrument from the list.

If you do not have an account yet or you do not have authorization please contact Mr. Thorsten Seidel (see also 'Contact')
 

Login für die Buchung:

http://web.biologie.uni-bielefeld.de/limitec-booking/

Bitte wählen Sie ein Gerät aus der Liste.

Falls Sie noch keinen Account haben oder Ihnen die Berechtigung fehlt, wenden Sie sich bitte an Herrn Thorsten Seidel (siehe auch 'Contact')

Technical Support

Speaker

Prof. Dr. Christian Kaltschmidt

Durch den Rektor ernannt: Standortvertreter für das Kompetenznetzwerk Stammzellenforschung

Telephone
+49 521 106-5625
Telephone secretary
+49 521 106-5797
Room
UHG W7-134


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