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LiMiTec

Light Microscopy Technology Platform of Bielefeld University

© Universität Bielefeld

Confocal Microscopes

Zeiss LSM780

Inverted confocal laser scanning microscope & FCS (W01)

  • Laser lines: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
  • Detectors: 2 photomultipliers, 32-ch GaAsp-detector
  • Optional: temperature-controlled stage with CO2-incubation
  • Software: Zeiss ZEN 2011

Phone: 5615

Leica STELLARIS 8 FALCON

Inverted confocal laser scanning microscope & FLIM & FCS (W0-226)

  • Laser lines: 405 nm, pulsed WLL 440 - 790 nm
  • Detectors: 2 HyD S, 2 HyD X, 1 HyD R
  • Optional: temperature-controlled stage with CO2-incubation
  • Software: Leica LAS X

Phone: 5690

Leica SP2 with Lambert LIFA (FLIM)

Upright confocal laser scanning microscope (W1)

  • Laser lines: 458 nm, 476 nm, 488 nm, 514 nm, 543 nm, 633 nm
  • Filtersets: DD458/514, DD488/543, TD488/543/633, RSP500
  • Detectors: 3 photomultipliers
  • Optional: temperature-controlled stage
  • Software: Leica LCS

Upright stand FLIM (frequency domain)

Lambert LIFA with multi-LED (485 nm, 540 nm, 635 nm)

Filtersets for Fluorescein (long pass), Rhodamin (long pass) and GFP (short pass)

 

Phone: 12706

Zeiss LSM 5 Exciter

Inverted confocal laser scanning microscope (W01)

  • Laser lines: 458 nm, 488 nm, 514 nm, 543nm, 633 nm
  • Filter: LP 475nm, LP 505nm, LP 530nm, LP 560nm, LP 650nm
  • Software: Zeiss ZEN 2008

Phone: 5615

Leica SPE

Confocal laser scanning microscope (AG Niehaus)

  • Lasers: 488 nm, 555 nm, 635 nm
  • Software: Leica LASAF

Light Sheet Microscope

Bruker Luxendo MuVi SPIM

Light sheet microscope for live imaging and cleared samples

  • Laser lines: 405 nm, 488 nm, 515 nm, 561 nm, 642 nm
  • 2 Hamamatsu Orca Flash cameras, separated by beam splitter
  • 4 Octagons for various applications

Fluorescence Microscopes

Leica Thunder Imager Tissue

Leica DM6B motorized upright fluorescence microscope  (W0-220)

  • Light source: coolLED pE300
  • Objectives: 5x/0.15, 10x/0.32 Ph, 20x/0.55, 40x/0.80, 100x/1.32 Oil
  • Filter sets: DAPI, GFP, Texas Red, Cy5
  • Cameras: Leica K5 and Leica K3C
  • Software: Leica LAS X with Thunder module

Nikon Eclipse 80i

Upright fluorescence microscope with Imagesplitter ratiometric for Ca2+-imaging (AG Niehaus)

  • Filter sets: INDO-1, DAPI, GFP, FITC, TRITC

Olympus ScanR

Inverted fluorescence microscope (IX81) for high-content screening (AG Niehaus)

  • Filter sets: UV1, UV2, CFP, GFP, YFP, Cy

Zeiss Axioskop2 (W5-258B)

Upright fluorescence microscope (AG Dietz)

  • Light source: 100 W HBO
  • Filter sets: DAPI (LP), GFP/FITC (LP), TRITC (LP), CFP (BP), YFP (BP), mCherry (BP)
  • Camera: Zeiss ICc1
  • Software: Axiovision release 4.7

Zeiss Axioskop

Upright fluorescence microscope (AG Kaltschmidt)

  • Light source: Carl Zeiss HBO 50
  • Filter sets: DAPI, GFP/FITC/A488, RFP/PE/A555
  • Camera: Zeiss MC100 Spot or Nikon consumer camera

Zeiss Axiovert25 (W5-220)

Inverted fluorescence microscope (AG Dietz)

  • Light source: 50 W HBO
  • Filter sets: DAPI (LP), GFP/FITC (LP)
  • Camera: Zeiss ERc5s
  • Software: ZEN LE

Zeiss Axiovert40 (W1-214)

Inverted fluorescence microscope

  • Light source: 50 W HBO
  • Filter sets: DAPI (LP), GFP/FITC (BP)
  • Camera: Nikon consumer camera

Zeiss Axiophot

Upright fluorescence microscope (AG Kaltschmidt)

  • Light source: Carl Zeiss HBO 50
  • Filter sets: DAPI, GFP/FITC/A488, RFP/PE/A555
  • Camera: Canon consumer camera

Microscopes, Polarization

Polarization Microscopy allows for visualization of birefringent structures such as cell walls, starch, spindles.

Octax PolScope

Polarization microscope (W01-236)

  • Inverted stand: Nikon Eclipse TE2000-S
  • Contrast methods: transmitted light (polarisator & analysator)
  • Software: Octax Eyeware
  • Camera: Octax camera system
  • Applications: imaging of birefringerant structures (spindles, cell walls)

Key-References:

Eichenlaub-Ritter, U., Winterscheid, U., Vogt, E., Shen, Y., Tinneberg, H.R., Sorensen, R. (2007) 2-methoxyestradiol induces spindle aberrations, chromosome congression failure, and nondisjunction in mouse oocytes. Biol. Reprod. 76(5): 784-793

Shen, Y., Betzendahl, I., Sun, F., Tinneberg, H.R., Eichenlaub-Ritter, U. (2005) Non-invasive method to assess genotoxiity of nocodazole interfering with spindle formation in mammalian oocytes. Reprod. Toxicol. 19(4): 459-471

Zeiss Axioplan

Polarization & Fluorescence microscope (W01-236)

  • Upright stand, filter sets for DAPI and FITC, phase contrast
  • Camera: Zeiss AxioCam MRm
  • Software: Axiovision

Microscopes, transmitted light

Evos XL

Advanced transmitted light inverted microscope (AG Kaltschmidt, AG Dietz)

  • Light source: LED
  • Contrast methods: transmitted light (brightfield&phase contrast)
  • Software: Evos
  • Camera: high sensitivity interline CMOS color camera
  • Applications: time-lapse, cell counting, cell viability assays, stem cell growth and differentiation, stem cell passaging, H&E imaging, DAB and other methods requiring true color images.

Olympus

Transmitted light inverted microscope (AG Kaltschmidt)

  • Contrast methods: transmitted light (brightfield & phase contrast)
  • Camera: Nikon consumer camera

Zeiss Axioplan

Upright transmitted light microscope (AG Kaltschmidt)

  • Contrast methods: : transmitted light (brightfield&phase contrast)
  • Camera: Optional MC100 Spot or Nikon consumer camera

Micro-Manipulation Setup

Zeiss Axiovert40CFL & Eppendorf Femtojet

Inverted Stand, fluorescence microscope (W01-250, AG Dietz)

  • Filter sets: CFP, YFP and FRET (CFP-YFP)
  • Camera: Zeisss MRm
  • Software: Zeiss Axiovision
  • Equipped with Eppendorf micro-injector

Stereo Microscopes

Leica MZFLIII

Fluorescence stereo microscope (AG Niehaus)

  • Filters sets: CFP, GFP, YFP, DsRed

Leica MZ6 (W5-258B)

Stereo microscope (AG Dietz)

  • Light source: Goose neck halogen illuminator
  • Camera: Nikon consumer camera

Leica MZ6 (W7)

Stereo microscope (AG Kaltschmidt)

  • Light source: transmitted light base
  • Nikon consumer camera (optional)

Leica MZ6 (W1-202)

Stereo microscope

  • Light source: transmitted light base
  • Nikon consumer camera (optional)

Zeiss SV8

Stereo microscope (AG Kaltschmidt)

  • Light source: transmitted light base
  • Nikon consumer camera (optional)

Wild R38

Stereo microscope (W01-236)

  • Light source: transmitted light base
  • Nikon consumer camera (optional)

Other Equipment

Clean bench and CO2-incubators

Available for LiMiTec-users in W01. Please contact Dr. Thorsten Seidel.

  • For live cell imaging, small mobile incubators and a temperature-controlled stage can be obtained on request.

External Instruments

ONI Nanoimager

Single-molecule localization microscopy (R2, 3. Etage)

  • Laser lines: 405 nm, 488 nm, 561 nm, 640 nm
  • Objective 100x/1.45
  • 2 simultaenous channels (dichroic mirror splitter, 640 nm)
  • Hamamatsu Orca Flash4.0 v.3
  • lateral resolution of 20 nm
  • suitable for single molecule localization microscopy, TIRF and single particle tracking

Prof. Dr. Sven Thoms

Biochemie und Molekulare Medizin

Medizinische Fakultät OWL

Phone: 68502

 

Die Anschaffung des Mikroskops war möglich durch eine Sachspende der Marlies und Herbert Repkow Stiftung.

Atomic Force Microscopes

Omicron STM/AFM

  • operated in dynamic mode under ultrahigh vacuum conditions

Bruker AFMs (modified)

  • for atomic-resolution imaging at the solid-liquid interface
  • solvation layer mapping is possible with home-built hard- and software

 

Prof. Dr. Angelika Kühnle

Physikalische Chemie I

Fakultät für Chemie

Phone: 2045

 

Referenzen:

https://pubs.acs.org/doi/10.1021/acs.jpcc.1c06213
https://doi.org/10.3762/bjnano.11.74
https://doi.org/10.1103/PhysRevB.100.205410
https://doi.org/10.1021/acs.langmuir.6b03814
https://doi.org/10.1063/1.4952954

 

Helium Ion Microscope (XPhy E0-302)

Zeiss Helium Ion Microscope

 

Dr. André Beyer

Physik supramolekularer Systeme und Oberflächen, Fakultät für Physik

Phone: 5364

Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Lifetime Imaging (FLIM)

Prof. Dr. Thomas Hellweg

PC III, Faculty of Chemistry

Phone: 6888

Coherent Raman Scattering (CARS) and Spontaneous Raman Scattering

Prof. Dr. Thomas Huser

Biomolecular Photonics, Faculty of Physics

Phone: 5450

Superresolution Microscopy (Nanoscopy)

Prof. Dr. Thomas Huser

Biomolecular Photonics, Faculty of Physics

Phone: 5450

Electron Microscopy

Prof. Dr. Andreas Hütten

Thin Films and Physics of Nanostructures, Faculty of Physics

Phone: 5412

 

Prof. Dr. Karsten Niehaus

Proteome and Metabolome Research, Faculty of Biology

Phone: 5631

 

Dr. Thorsten Seidel

CF Microscopy and Imaging (under construction)

Phone: 5588

Cryo-Electron Microscopy

Prof. Dr. Andreas Hütten

Thin Films and Physics of Nanostructures, Faculty of Physics

Phone: 5412

 

Prof. Dr. Thomas Hellweg

PC III, Faculty of Chemistry

Phone: 6888

Atomic Force Microscopy

Prof. Dr. Dario Anselmetti

Biophysics and Nanoscience, Faculty of Physics

Phone: 6870

 

Prof. Dr. Angelika Kühnle

PC1, Faculty of Chemistry

Phone: 6887

Image Analysis

Bio Data Mining Group

Field of research / key experience: Development of computational approaches to harvest bioimage data.

Keywords: Bioimage Informatics, Computer Vision, Machine Learning, Remote Sensing, Medical Image Analysis, Information Visualization

Available resource: Online image annotation platform BIIGLE (www.biigle.de) for manual and AI-assisted image / video annotation (development since 2009, > 4,000 users).

 

Prof. Tim W. Nattkemper (tim@biigle.de)

www.uni-bielefeld.de/fakultaeten/technische-fakultaet/arbeitsgruppen/biodata-mining

Faculty of Technology

Bielefeld University

 

References

BIIGLE 2.0 - Browsing and Annotating Large Marine Image Collections. Langenkämper D, Zurowietz M, Schoening T, Nattkemper TW. Frontiers in Marine Science, 4,  2017, 83, DOI=10.3389/fmars.2017.00083, ISSN=2296-7745

Current trends and future directions of large scale image and video annotation: Observations from four years of BIIGLE 2.0. M Zurowietz, TW Nattkemper, FRONTIERS IN MARINE SCIENCE (section Ocean Observation), 2021, Manuscript ID: 760036A, https://doi.org/10.3389/fmars.2021.760036

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