LiMiTec

Zeiss LSM780

Inverted confocal laser scanning microscope & FCS (W01)

• Laser lines: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
• Detectors: 2 photomultipliers, 32-ch GaAsp-detector
• Optional: temperature-controlled stage with CO2-incubation
• Software: Zeiss ZEN 2011

Phone: 5615

Zeiss LSM 900

Inverted confocal laser scanning microscope (Building R1-D2)

• Laser lines: 405 nm, 488 nm, 561 nm, 640 nm
• 2 Detectors
• Temperature-controlled with CO2-incubation
• Software: Zeiss ZEN Blue

Phone: xxxx

Spinning Disc Nikon Ti2E Crest X-Light V3

Inverted Stand Nikon Ti2E with Crest Spinning Disc (Building R1-D2)

• Objectives: 10x/0.45, 20x/0.8, 40x/0.95, 40x/1.15, 60x/1.42
• Laser lines: 405 nm, 446 nm, 476 nm, 518 nm, 546 nm, 637 nm, 748 nm
• Camera: Photometrix Kinetics
• Temperature-controlled with CO2-incubation
• Nikon NIS Elements

Phone: xxxx

Leica SP2 mit LIFA FLIM

Upright confocal laser scanning microscope (W1)

Laser lines: 458 nm, 476 nm, 488 nm, 514 nm, 543 nm, 633 nm
Filtersets: DD458/514, DD488/543, TD488/543/633, RSP500
Detectors: 3 photomultipliers
Optional: temperature-controlled stage
Software: Leica LCS

Upright stand FLIM (frequency domain)

Lambert LIFA with multi-LED (485 nm, 540 nm, 635 nm)

Filtersets for Fluorescein (long pass), Rhodamin (long pass) and GFP (short pass)

Phone: 12706

Zeiss LSM 5 Exciter

Inverted confocal laser scanning microscope (W01)

• Laser lines: 458 nm, 488 nm, 514 nm, 543nm, 633 nm
• Filter: LP 475nm, LP 505nm, LP 530nm, LP 560nm, LP 650nm
• Software: Zeiss ZEN 2008

Phone: 5615

Bruker Luxendo MuVi SPIM

Light sheet microscope for live imaging and cleared samples

• Laser lines: 405 nm, 488 nm, 515 nm, 561 nm, 642 nm
• 2 Hamamatsu Orca Flash cameras, separated by beam splitter
• 4 Octagons for various applications

Leica Thunder Imager Tissue

Leica DM6B motorized upright fluorescence microscope  (W0-220)

• Light source: coolLED pE300
• Objectives: 5x/0.15, 10x/0.32 Ph, 20x/0.55, 40x/0.80, 100x/1.32 Oil
• Filter sets: DAPI, GFP, Texas Red, Cy5
• Cameras: Leica K5 and Leica K3C
• Software: Leica LAS X with Thunder module

Keyence BZ-X800

Mobile Inverted Fluorescence Microscope for Teaching

Objectives: 5x, 10x, 25x, 40x LD PH, 60x

Filter sets: DAPI, FITC, RHOD

Light source: HXP-lamp

Polarization Microscopy allows for visualization of birefringent structures such as cell walls, starch, spindles.

Octax PolScope

Polarization microscope (W01-236)

• Inverted stand: Nikon Eclipse TE2000-S
• Contrast methods: transmitted light (polarisator & analysator)
• Software: Octax Eyeware
• Camera: Octax camera system
• Applications: imaging of birefringerant structures (spindles, cell walls)

Key-References:

Eichenlaub-Ritter, U., Winterscheid, U., Vogt, E., Shen, Y., Tinneberg, H.R., Sorensen, R. (2007) 2-methoxyestradiol induces spindle aberrations, chromosome congression failure, and nondisjunction in mouse oocytes. Biol. Reprod. 76(5): 784-793

Shen, Y., Betzendahl, I., Sun, F., Tinneberg, H.R., Eichenlaub-Ritter, U. (2005) Non-invasive method to assess genotoxiity of nocodazole interfering with spindle formation in mammalian oocytes. Reprod. Toxicol. 19(4): 459-471

Zeiss Axioplan

Polarization & Fluorescence microscope (W01-236)

• Upright stand, filter sets for DAPI and FITC, phase contrast
• Camera: Zeiss AxioCam MRm
• Software: Axiovision

Zeiss Axiovert40CFL & Eppendorf Femtojet

Inverted Stand, fluorescence microscope (W01-250, AG Dietz)

• Filter sets: CFP, YFP and FRET (CFP-YFP)
• Camera: Zeiss MRm
• Software: Zeiss Axiovision
• Equipped with Eppendorf micro-injector

FACS Sony SH800

Cell Sorter

Laser lines 405 nm, 488 nm, 638 nm

Nozzle sizes: 70 µm, 100 µm, 130 µm

Cell Box

portable Incubator for mammalian cells

Clean bench and CO2-incubators

Available for users. Please contact Dr. Thorsten Seidel (UHG) or Dr. Barbara Biermann (R1-D2).

• For live cell imaging, small mobile incubators and a temperature-controlled stage can be obtained on request.

ONI Nanoimager

Single-molecule localization microscopy (R2, 3. Etage)

• Laser lines: 405 nm, 488 nm, 561 nm, 640 nm
• Objective 100x/1.45
• 2 simultaenous channels (dichroic mirror splitter, 640 nm)
• Hamamatsu Orca Flash4.0 v.3
• lateral resolution of 20 nm
• suitable for single molecule localization microscopy, TIRF and single particle tracking

Prof. Dr. Sven Thoms

Biochemie und Molekulare Medizin

Medizinische Fakultät OWL

Phone: 68502

Die Anschaffung des Mikroskops war möglich durch eine Sachspende der Marlies und Herbert Repkow Stiftung.

Atomic Force Microscopy

Omicron STM/AFM

operated in dynamic mode under ultrahigh vacuum conditions

Bruker AFMs (modified)

for atomic-resolution imaging at the solid-liquid interface
solvation layer mapping is possible with home-built hard- and software

Prof. Dr. Angelika Kühnle

Physikalische Chemie I

Fakultät für Chemie

Phone: 2045

Referenzen:

https://pubs.acs.org/doi/10.1021/acs.jpcc.1c06213
https://doi.org/10.3762/bjnano.11.74
https://doi.org/10.1103/PhysRevB.100.205410
https://doi.org/10.1021/acs.langmuir.6b03814
https://doi.org/10.1063/1.4952954

Zeiss Helium-ion Microscope

Contact: Dr. André Beyer

Physik supramolekularer Systeme und Oberflächen, Fakultät für Physik

Phone: 5364

Bio Data Mining Group

Field of research / key experience: Development of computational approaches to harvest bioimage data.

Keywords: Bioimage Informatics, Computer Vision, Machine Learning, Remote Sensing, Medical Image Analysis, Information Visualization

Available resource: Online image annotation platform BIIGLE (www.biigle.de) for manual and AI-assisted image / video annotation (development since 2009, > 4,000 users).

Prof. Tim W. Nattkemper (tim@biigle.de)

www.uni-bielefeld.de/fakultaeten/technische-fakultaet/arbeitsgruppen/biodata-mining

Faculty of Technology

Bielefeld University

References

BIIGLE 2.0 - Browsing and Annotating Large Marine Image Collections. Langenkämper D, Zurowietz M, Schoening T, Nattkemper TW. Frontiers in Marine Science, 4,  2017, 83, DOI=10.3389/fmars.2017.00083, ISSN=2296-7745

Current trends and future directions of large scale image and video annotation: Observations from four years of BIIGLE 2.0. M Zurowietz, TW Nattkemper, FRONTIERS IN MARINE SCIENCE (section Ocean Observation), 2021, Manuscript ID: 760036A, https://doi.org/10.3389/fmars.2021.760036